The book ‘Biophysical Chemistry’ provides a pedagogical account of diverse topics of biophysical chemistry ranging from the structure of cells, lipids and proteins to nonequilibrium thermodynamics, enzyme catalysis and fast reaction kinetics. For convenience, the book is divided into two parts viz (i) Biomolecules and (ii) Biochemical Techniques. In order to cater to the needs of diverse spectrum of readers, the emphasis has been on the fundamental structural concepts in the first part (Chapters 1 to 12) while the second part (Chapters 13 to 32)provides a systematic account of different experimental techniques such as chromatography, electrophoresis, gel filtration etc that are extensively employed in biochemical research. A few typical applications using NMR, ESR and SPR are provided so as to comprehend the power of spectroscopic tools in biophysical chemistry. Starting from an elementary treatment of structural organic chemistry, the book deals with advanced topics of entropy production and biosensors. The book consists of a large number of schematic diagrams as well as tabular compilations of kinetic, electrochemical and thermochemical data. In view of the diverse coverage of topics, this book will be a valuable asset to undergraduate and post graduate students of chemistry, biology and medicine. An unique feature of the book is the inclusion of the multiple choice questions, numerical examples and descriptive questions in various chapters. Contents: Part-I Different Types of Biomolecules 1. Cell and its biochemical setup 2. Thermodynamic aspects of the cell 3. Carbohydrates, their reactions, thermochemistry and energetics 4. Lipids 5. Amino acids 6. Peptides 7. Proteins 8. Nucleosides and nucleotides 9. Enzymes 10. Co-enzymes and Vitamins 11. Bioenergetics 12. Biosensors Part-II Bioanalytical techniques 13. Surface Plasmon resonance spectroscopy 14. Affinity chromatography 15. Capillary Electrophoresis 16. NMR technique in the elucidation of biochemical problems 17. Applications of ESR technique for the solution of biological problems. 18. Flow methods for the kinetic study of fast biochemical systems. 19. Temperature jump relaxation technique for the kinetic study of fast biochemical reactions 20. Flash photolysis technique for the kinetic study of fast biochemical reactions 21. Pressure Jump relaxation method for the study of fast biochemical reactions 22. Circular dichroism as a tool for the analysis of biochemical reactions 23. Applications of isothermal calorimetry in the study of biochemical reactions 24. Principles of differential scanning calorimetry and its applications to the study of biochemical reactions 25. Applications of gel filtration technique in the separation of biomolecules 26. Principles of gel electrophoresis and its applications to biochemical analysis 27. Analytical ultracentrifugation method in the analysis of biomolecules 28. Ion exchange chromatography and its applications in the separation of biomolecules 29. Principles of surface enhanced Raman scattering and its bio analytical applications 30. Mass spectroscopy and its applications in the analysis of biomolecules 31. X-ray studies in the elucidation of the structure of bio molecules 32. CRISPR –CAS9- A method for genome editing About the Authors: Dr C Kalidas served as a Faculty at the Department of Chemistry IIT Madras, for more than three decades and was also an Emeritus Scientist (CSIR) at IIT Madras. He visited the Max-Planck Institute for Physical Chemistry(later Max-Planck Institute for Biophysical chemistry)Gottingen,Germany as a German Academic Exchange scholar in 1968-69 and again as a senior fellow of Alexander von Humboldt Foundation during 1976-77 and 1990-91.His research interests are centred around electrochemistry, fast reaction kinetics and macrocyclic chemistry. He was also an IUPAC committee member for analytical chemistry. He has taught a variety of chemistry courses at undergraduate and post graduate levels at IIT Madras. Dr M V Sangaranarayanan obtained his Ph D from the Indian Institute of Science, Bangalore and was an Alexander von Humboldt Fellow at the University of Bonn, Germany. He has been a Faculty at the Department of Chemistry IIT Madaras from 1993 onwards till date. His research interests include non-equilibrium thermodynamics, interfacial electrochemistry, and supercapacitors. He has published 125 papers in refereed international journals and co-authored three textbooks. He has taught diverse theory courses at IIT Madras, ranging from Electrochemistry and statistical thermodynamics to analytical chemistry and Biomolecules. He is the recipient of the Prof C N R Rao National Prize for research in Chemical Sciences and was the President, Society for the Advancement of Electrochemical Science and Technology, India.