The present book “General Veterinary Microbiology” is divided into four parts and twenty three chapters. The Part I includes general bacteriology, which deals with the history, microscopy, classification, nomenclature, structure, morphology, nutrition, growth, cultivation, sterilization, disinfection, identification, antibiotic resistance and genetics of bacteria. The part II provides introduction to fungi. The part third includes general virology, which deals with the introduction, classification, cultivation, virus-cell interaction, interferon and genetics of animal viruses. The fourth part relates to bacterial aspect which includes microbial staining techniques, demonstration of fungi, bacterial motility and determination of phenol coefficient. Glossary, highlights and multiple choice questions embedded at the end of the book enables the readers to understanding the subject matter in depth. This book has been prepared according to the latest syllabus approved by VCI for B.V.Sc. & A.H. degree programme. This is a valuable source book for the students of veterinary microbiology and will also be useful for veterinary disease investigators and practitioners of veterinary science.