The present manual has been designed in accordance with the recommendations of New MSVE (Minimum Standards of Veterinary Education) course outline for B.V. Sc. & A. H. graduates. The manual includes Veterinary Mycology, Veterinary Immunology & Serology and Microbial Biotechnology. The text features detailed experimental protocols with comprehensive sections on materials and preparations for all exercises. This comprehensive manual is a reference guide to students which covers various aspects of Veterinary Microbiology ranging from the simplest Lacto phenol Cotton Blue Staining, various immunological techniques such as agglutinations, precipitations, ELISA etc. to various molecular techniques such as PCR, SDS-PAGE etc. It fulfils the fundamental needs of the students while studying Veterinary Microbiology. While the manual is designed for veterinary graduates, it can be recommended for any laboratory or institution involved in diagnostic or research work.