Plant biology has emerged as most important addition to conventional crop improvement and in research laboratory programmes. However, advanced techniques to study and work in the field of plant biology existed only from few decades. Our manual “Principles and Methods in Plant Biology” has been presented in a manner that will allow even a novice reader appreciate and understand the theory and perform advanced protocols and techniques in plant biology. Various chapters in this manual include information pertaining to fractionalization procedures, separation procedures, estimation procedures, staining procedures, tissue culture procedures, genetic marker procedures, DNA markers types and their development, markers assisted selection, basic plant biology manipulations, biochemical and phytochemical analysis information etc. We hope that following these working protocols and understanding the theoretical bases behind them will benefit your research.