Contents: 1. Mapping Populations and their Applications. 2. Polymerase Chain Reaction (PCR): Types and Applications. 3. Molecular Markers for Crop Improvement. 4. Applications of RAPD and ISSR Markers in Plant Genome Analysis. 5. Usefulness of Microsatellite as Molecular Markers. 6. Plant Breeder's Right. 7. Plant Tissue Culture: History and Basic Principle. 8. Plant Breeding: Haploid Culture and Production. 9. Antinutritional Factors in Pulses and their Diversity. 10. Basic Techniques of DNA Analysis. Plant science is one of the fundamental subjects to begin with. Biotechnology has given it a force to get modified into an applied field known as plant biotechnology. Plant tissue culture is widely used for direct commercial applications as well as value in basic research into cell biology, genetics and biochemistry. Metabolic engineering of plants promises to create new opportunities in agriculture, environmental applications, production of chemicals and even medicine. Therefore, molecular techniques encompassing the use of plants are being focused for the generation of new promising applications in this era. The main aim of this book is to provide students and researchers about the importance and opportunities available in plant biotechnology. Plant biotechnology now allows the transfer of a greater variety of genetic information in a more precise and controlled way. The potential for improving plant productivity and its proper use in agriculture relies largely on newly developed molecular markers. A number of methods have been developed and validated in association with the use of genetically transferred cultures in order to understand the genetics of specific plant traits. Such relevant methods can be used to determine the markers that are retained in genetically manipulated organisms and to determine the elimination of marker genes. These techniques enable the selection of successful genotypes, better isolation and cloning of favourable traits, and the creation of transgenic organisms of importance to agriculture and industry. The book describes the basic aspects of plant biotechnology and IPR. The impact of biotechnology on crop improvement is introduced with the help of suitable examples. The book format is designed in order to help the reader to grasp and understand the complexity of plant biotechnology. The aim of this book is to serve as a comprehensive plant biotechnology textbook as well as a wide-ranging reference book and impart high value to advanced undergraduate and graduate students, and research scholars.