The book “TECHNIQUES IN MOLECULAR BIOLOGY AND PLANT BIOTECHNOLOGY” is a compendium on the laboratory experiments in molecular biology, plant tissue culture, genetic engineering and immuno-diagnostics covering a total of 90 experiments. The present day education system focuses on skilling and development of entrepreneurial human resources. Biotechnology has emerged as a promising career option demanding skilled biotechnologists in various sectors like agriculture, horticulture, animal sciences, fisheries science, natural resource management, medicine, pharmaceutical and food processing industries. The step by step procedure on different techniques in plant biotechnology presented in the book will be an authentic knowledge source and a ready reckoner for skill and capability development in biotechnology for students, research scholars, teachers and scientists. Contents: Section 1. Techniques in Molecular Biology Deepu Mathew, Donald James, S. Binu, Mariet Jose Rehna Augustine, Smita Nair and M.R.Shylaja Section 2. Immunology and Molecular Diagnostics S. Binu and Smita Nair Section 3. Plant Tissue Culture and Its Applications M.R. Shylaja, Mariet Jose, Donald James and Deepu Mathew Section 4. Plant Genetic Engineering Donald James and Rehna Augustine About the Authors: Prof. (Dr.) M.R. Shylaja did her Masters and Doctorate degrees in Horticulture from Kerala Agricultural University (KAU), Thrissur, India . Prof. Shylaja has 34 years of service at KAU. She served in the capacity of Director of Education, KAU & KAU- ICAR Education Nodal Officer. She also headed the Department of Plant Biotechnology (Centre for Plant Biotechnology and Molecular Biology), College of Horticulture, KAU. Dr. Deepu Mathew is working as Assistant Professor at Kerala Agricultural University, Thrissur, India. At Centre for Plant Biotechnology and Molecular Biology, KAU, he is working in horticultural crop genomics, transcriptomics and bioinformatics. Prof. (Dr.) K. V. Peter is an alumnus of G.B. Pant University of Agriculture and Technology, Pantnagar. He did postdoctoral research at Vegetable Laboratory, USDA, Beltsville and at AVRDC (World Vegetable Centre), Taiwan. He was Professor of Horticulture at Kerala Agricultural University and served in the capacities of Director, ICAR-IISR, Calicut and Vice-Chancellor, Kerala Agricultural University.